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The analytical technique in that study (liquid chromatographyisotopic dilution mass spectrometry) more closely resembles the LCMS/MS assay we used than does the HPLC technique used by Borgstrm and colleagues in their 1986 study [4]

The analytical column used was a silica-based reversed-phase ACQUITY UPLC M-Class Peptide BEH C18 Column, 75 m 150 mm, 1.7 m particle size and 130 pore size (Waters)

The problem is levels drop with age, stress, and poor diet faster than most people realise

Nucl Acid Res 1986

Interleukin-6 regulates iron-related proteins through C-Jun N-terminal kinase activation in Bv2 microglial cell lines

This variability may be attributed to curcumins limited bioavailability, as it is rapidly metabolized and excreted from the body (493, 494)