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Current research often focuses on isolated signature molecules (e.g., GPX4, SLC7A11, FDX1) within genetically homogeneous cell lines, which fail to recapitulate the metabolic heterogeneity and complex stromal interactions of the in vivo tumor microenvironment (TME)

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After 24 h, the biofilm was washed once with 1 PBS and imaged using phase contrast microscopy (Zeiss, Axio, Germany), for bacterial adhesion and colonization