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Further evidence was provided by a time-course western blot analysis of striatal GSTO2 and TH protein levels, showing GSTO2 upregulation before TH increase by 1 month (Figs

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Agar gels were mixed with 1 mL PBS by vigorous pipetting before the addition of calcein-AM, DCFDA, or mBcl to the same concentration as indicated for cells cultured in liquid medium and incubated for 30 min in the dark at the temperature indicated for those cells