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A 0.5C charge rate would take approximately two hours, and a 2C charge rate would complete in about 30 minutes, though with much higher thermal stress

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Samples were homogenized for 2 min at 30 Hz with TissueLyser II (Qiagen), then underwent three freeze-thaw cycles before removing debris with centrifugation at 2000 rpm for 15 min at 4 C, and supernatant quantified and stored frozen

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Artificial sweeteners Artificial sweeteners can disrupt the bodys natural production of glutathione, leading to lower levels of this important antioxidant