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For non-reducing SDS-PAGE, 2 g of protein was mixed with 3 L of 4 Bolt LDS Sample Buffer (Invitrogen) and Milli-Q water to a total volume of 12 L

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3.2.1.2 Semaglutide and Sodium N-(8-[2-Hydroxybenzoyl] Amino) Caprylate (SNAC) Pharmacokinetic parameters for semaglutide and SNAC are shown in ESM Online Resource 1, Table S3a