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To set up the assay, tubes with 0.1 mL distilled water, standard solution and the treatment samples were prepared, and then PBS and nitrate reductase were added into each tube

In addition, alterations in reproductive hormone levelsincluding decreased testosterone and increased LH/FSH ratioshave been documented in diabetic men, further supporting the endocrine disruption described in experimental studies [164, 302]

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Peptide degradation occurs when temperature and time exceed guidelines