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doi: 10.1016/s0304-3940(00)01699-2

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As was previously mentioned, levels of GH and IGF-1 steadily decrease over a lifespan, and with the lower hormone levels comes a myriad of side effects such as increased fat mass, cardiovascular dysfunction, decreased muscle mass, and declined cellular protein synthesis

Antibodies and Dyes for Flow Cytometry Antibodies were used at manufacturer recommended concentrations and cells were incubated at 4C for 25 mins prior to washing and acquisition: CD3 FITC (BD Biosciences), CD56 PE (BD Biosciences), CD16 PE-Cy7 (ThermoFisher), SLAMF7 PE (BioLegend), and NKG2D APC (ThermoFisher)