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10.1007/s00018-017-2547-4 Cell

In younger cells, FOXO4 appeared to be mostly in the cytoplasm, while in older cells it was more often in the nucleus, which may be considered its more active form

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Such conditions occur through minor variations in elevation in waterlogged and flooded natural landscapes, and in some engineered wetlands (e.g., ridge and furrow, raised fields, floating fields) (Figure 2D)

HPLC separates the components of a sample and measures what percentage is actually the target peptide versus impurities

You might feel a quick pinch or light pressure, but discomfort is minimal