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Purification of CTL1 for cryo-EM Membranes were thawed and resuspended in basis buffer supplemented with 5 mM choline chloride, 0.7% DDM, 0.07% CHS, and BioLock on a benchtop rotator at 4C for 1 h

The frequency of treatments depends on your specific goals and needs

If you experience headaches, rest and drink plenty of fluids

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While selenium is essential, excessive intake above 400mcg daily long-term can cause selenosis (selenium toxicity), with symptoms including hair loss, nail brittleness, nausea, and fatigue

Images were taken with a Zeiss LSM710 confocal microscope and quantified using Fiji ImageJ