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7-Dichlorofluorescin Diacetate (DCFDA) Assay for Production of Reactive Oxygen Species Cells were incubated in 10 M DCFDA (Sigma) in PBS at 37C for 30 min as previously described (Chiu et al., Quantification of 8-Hydroxy-2-Deoxyguanosine (8-OH-dG) as a Marker for Oxidative DNA Damage We used 8-Hydroxy-2-Deoxyguanosine (8-OH-dG) as a biomarker for oxidative DNA on brain cells exposed to different treatments using the commercially available HT 8-OH-dG ELISA II kit (R&D Systems) as guided by the manufacturers instructions

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Analysis of supportive secondary endpoints: Semaglutide 2.4 mg was also associated with improvements in multiple cardiovascular risk factors, including waist circumference, glycated haemoglobin level, systolic and diastolic blood pressure, high-sensitivity C-reactive protein and lipids

Further, our lab previously validated an in vitro EEC model that recapitulated the primary features of our in vivo chronic paternal stress paradigm while allowing for cell-type specific EV collection 25

Intracellular transport and functional defects