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Although a natural enzyme can serve as an effective starting point for laboratory evolution, directed evolution experiments struggle when mutations that support the desired function incur excessive folding or solubility deficiencies 18,19,20,21

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Activation leads to: Increased satiety signalling in the hypothalamus Slowed gastric emptying Reduced appetite drive Improved insulin signalling This mechanism primarily affects energy intake rather than directly increasing energy expenditure

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Most tracking systems fail to provide meaningful context for the data