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Methylation-specific PCR (MSP) and dot blot Full methods are available in Supplementary Methods

Here are a few safe methods: Stool softeners: These are not the same as laxatives and can sometimes be used for a short time to help soften stool if it becomes too hard

(PubMed) Yin G, Ming H, Zheng X, Xuan Y, Liang J, Jin X

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If they occur, or are allowed to occur, they will determine the amount of bioavailable niclosamide that can permeate the mucin in a nasal spray or inhaled administration