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doi: 10.1016/j.cmet.2014.10.002

3,4-Dihydroxyphenylacetaldehyde-induced protein modifications and their mitigation by N-acetylcysteine

10.1111/bpa.12350 Brain Pathol

the lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

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238 However, other works have demonstrated that SIRT2 promotes cancer growth by stabilizing the Myc family of oncoproteins 239,240 and enhancing the signaling of the Notch pathway