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Ishikawa H, Imano M, Shiraishi O, Yasuda A, Peng Y-F, Shinkai M, et al

Conclusion: We have shown that illicit drugs used in PCT may be substituted, adulterated, or contain no active ingredients

While primers sequence for IL8, HSP27 and SOD1 were designed using Primer3 ( and Primer-BLAST ( Primer specificity was verified by running PCR for 40 cycles at 95 C for 10 seconds and 60 C for 60 seconds, followed by gel electrophoresis on a 3% agarose gel stained with SYBR safe DNA gel stain

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Arnaldi LA, Borra RC, Maciel RM, Cerutti JM