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In the brain, excess ferrous iron can potentially disrupt the activity of both DNA methyltransferases (DNMT) and ten-eleven translocation proteins (TET) enzymes, impairing the DNA methylation and demethylation processes that regulate gene expression (Zhao et al., 2014)

ApopTag Peroxidase kits (Merck Millipore, Bayswater, VIC, Australia) were used for TUNEL staining

More importantly, PKM2 can translocate into the nucleus, where it regulates the transcription of metabolic genes such as HIF-1, LDHA, and GLUT1, thereby enhancing glycolysis, suppressing OXPHOS, and establishing a metabolic firewall against therapeutic stress [97]

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Understanding these adjustment factors is a hallmark of responsible and effective peptide cycle planning