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Biochem Pharmacol 60 : 11011107 Clarke RB, Howell A, Anderson E (1997) Type I insulin-like growth factor receptor gene expression in normal human breast tissue treated with oestrogen and progesterone

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The membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature