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CRISPR-Cas9 is a construct consisting of a guide RNA (gRNA) that targets the genetic loci of interest and the Cas9 endonuclease enzyme, which functions as a pair of nucleotide scissors that cleave the DNA at the target siteeffectively generating a double-stranded DNA break for subsequent genome editing [51]

A key area of interest is its ability to influence the expression of over 4,000 human genes approximately 6% of the human genome as revealed through analysis using the Broad Institute's Connectivity Map

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