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25 4.1.13'CRISPR/Cas9' Table 15: gRNAs used for CRISPR/Cas9 gRNA forward reverse ATF4_2 CACCGTAATAAGCAGCCCCCCCAGA AAACTCTGGGGGGGCTGCTTATTAC ATF4_3 CACCGAGTCCCTCCAACAACAGCAA AAACTTGCTGTTGTTGGAGGGACTC Gclc_1 CACCGCAATATGAGGAAACGCCGGA AAACTCCGGCGTTTCCTCATATTGC Gclc_2 CACCGTATGGGAGTTACATGATCGA AAACTCGATCATGTAACTCCCATAAC All gRNAs were designed by BROAD Institute