40:69-76 [PubMed: 27797925] 273
GCL is specific for the glutamyl moiety and is regulated physiologically by: (a) non-allosteric feedback competitive inhibition by GSH (Ki=2.3mM), which involves binding of GSH to the glutamate and another site on the enzyme [8, 9] and (b) availability of its precursor, cysteine [1] .The apparent Km values of GCL for glutamate is 1.8 mM, and for cysteine and 0.10.3 mM [9]
The final solution should be completely clear, with no cloudiness, particles, or discoloration
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Plasma biomarker panel for major depressive disorder by quantitative proteomics using ensemble learning algorithm: a preliminary study
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