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Global analysis of the mitochondrial proteome in response to cellular GSH depletion To identify mitochondrial proteins regulated by GSH availability, we used quantitative proteomics and analyzed mitochondria immunopurified from HeLa cells expressing a mitotag (3XHA-OMP25-mCherry) grown in standard media or treated with the glutathione synthesis inhibitor, buthionine sulfoximine (BSO) 13
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The phospholipids from sunflower lecithin are what create the liposomal effect described above
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