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These may indicate acute cholecystitis, bile duct obstruction, or cholangitis requiring urgent intervention

After washing in PBS, horseradish peroxidase-conjugated goat anti-rabbit IgG (ab175470, Abcam) diluted 1:200 in 1% rabbit serum in PBS was applied, and the slides were incubated for 30 min at room temperature

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Individuals with severe gastrointestinal problems such as gastroparesis (delayed stomach emptying) or a history of severe pancreatitis (inflammation of the pancreas) should not use tirzepatide, as it can aggravate these conditions

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