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To restore NAD + levels, ATM-deficient models were treated with NR, which stimulated PINK1-dependent mitophagy, reduced cytoplasmic DNA levels, and activated the NAD + -dependent SIRT1 signaling pathway, which in turn further promoted mitochondrial mitophagy and healthy morphology 111,288

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[7] [9] These contraindications are based on known metabolic pathways, clinical case reports, and established pharmacological principles, and are supported by clinical guidelines and expert consensus

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Each dot represents one sgRNA, n = 10 for each gene