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There are also a number of health conditions that prevent patients from being able to absorb a sufficient amount of vitamin B12 both through their diet and through vitamin supplements, making injections the only method that ensures they are not deficient

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Upon loss of microbial signals, modeled by germ-free, gnotobiotic, and broad-spectrum antibiotic-treated rodents, eEANs sensed the changes in intestinal epithelial and mucosal areas in the lamina propria, relayed signals to sensory vagal afferents of the nodose ganglion and dorsal vagal complex, sympathetically activated glutaminergic neurons lateral to the paragigantocellular nucleus/rostral ventrolateral medulla (LPGi/RVLM) and downstream coeliac-superior mesenteric ganglia (CM-SMG), whereby efferent nerves reduced GI transit time

This can be due to having low levels of intrinsic factor in the digestive system, having a digestive condition, or following a plant-based diet