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Western blot analysis Western blotting was performed as previously described via the following antibodies: FBXO22, BACH1, KLF4, LKB1, SNAL1, PTEN, KDM4A, BAG3, and GAPDH (all from Proteintech, Rosemout, IL)

Long-acting opioids (methadone, extended-release formulations): Onset is slower, usually 24 to 48 hours after the last dose

Testosterone replacement therapy is not a set-it-and-forget-it prescription

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