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The protein:ligand complex was crystallized after 3 days of incubation at 20 C in 20 mM zinc chloride and 20% (w/v) PEG 3,350 by the hanging-drop vapor diffusion method (2 L protein-ligand complex:2 L crystallization solution)

In vitro, measurements revealed that the increase in cortical BMC was due to an increased cross-sectional bone area, whereas the cortical volumetric BMD was unchanged

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