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WAT mitochondria isolation To isolate mitochondria, fresh gWAT was minced then homogenized using a drill-operated Teflon pestle in ice-cold MSHE buffer (70 mM sucrose, 210 mM mannitol, 5 mM HEPES, 1 mM EDTA, pH 7.2) containing 0.5% FA-free BSA

doi: 10.1007/s10534-022-00447-9

10.1111/jnc.70073 [DOI] [PMC free article] [PubMed] [Google Scholar] Federici L., Masulli M., De Laurenzi V., Allocati N