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The next day, the brain slices were washed by the PBST (0.1% Triton X-100 in PBS) at least 15 min/three times to washout primary antibody

BPC stands for Body Protection Compound, and it is known for influencing the growth factors of the body, including helping the production of blood vessels and protecting the body against inflammation

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Results and discussion Structure determination We used NanoBiT strategy 22,23,24 to stabilize the complex, in which GLP-2R was directly attached to LgBiT and rat G was linked to HiBiT with a 15-amino acid linker in between