To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer
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Phase II takes these highly reactive, dangerous intermediates and binds (conjugates) them to endogenous molecules to neutralize them
Report any side effects promptly, and do not increase the dose on your own Conclusion: Starting low and titrating gradually for optimal results Clinical trials used multiple starting doses, but the safest, most common starting dose is 12 mg weekly
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