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A cornerstone of the research methodology hinged upon rigorous metabolic phenotyping and hormonal profiling among participants aged 12 to 35 years, ensuring that observed outcomes were robustly correlated with clinically significant weight loss

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Protein lysates were boiled at 95 C for 10 min on a thermomixer (Thermo Fisher Scientific) and sonicated on the Bioruptor (Diagenode) system

Power JM, Sah P (2007)

It should be taken daily on an empty stomach with no more than 4 ounces of water, at least 30 minutes before eating or drinking

The specific bacterial species present in the gut, particularly those in the genera Clostridium, Escherichia, and Proteus, determine conversion rates