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Despite these challenges, several phase 2 combination trials have been completed

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Defective hypothalamic autophagy and vesicular trafficking are also considered relevant pathogenic factors in the development of obesity 63,64

Cells were spun down at 3000 g for 5 min, the supernatant was discarded, the pellet was resuspended in 30 mL cold H 2 O and spun down as before described, supernatant was discarded