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International journal of molecular sciences, 24(23), 16588
Briefly, whole Drosophila were fixed in 4% PFA for 12 h at 4 C, then rinsed 3 PBS for 3 h at RT with gentle shaking, and then immersed in FlyClear Solution-1 (8% THEED (2,2,2,2- (Ethylenedinitrilo)-tetraethanol) (Sigma-Aldrich), 5% Triton X-100 (Sigma-Aldrich) and 25% Urea (Fisher Scientific, in PBS) for 14 days at 37 C, as described previously 26
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Genovese F, Manresa AA, Leeming DJ, Karsdal MA, Boor P
Researchers frequently cross-reference a peptide half-life chart when selecting between modified and unmodified GHRH analogs precisely because this timing difference significantly affects experimental outcomes in hypothalamic-pituitary axis studies
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