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On the left is taken from a young animal and on the right is taken from an old animal

New UV exposure creates fresh pigment faster than any active can fade the old, which is why sunscreen is not optional alongside a brightening routine in South Africa

Sections underwent deparaffinization in xylene, rehydration through graded ethanol series, and heat-induced antigen retrieval in citrate buffer (pH 6.0, 95 C, 10 min), followed by blocking with 3% bovine serum albumin (BSA) for 30 min at room temperature

PMID 8185623

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Consult your doctor for advice in such situation