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If you are already following a healthy diet, exercising regularly, and sleeping well, your preparation time may be shorter

doi: 10.1016/j.jogoh.2024.102723 61 BuggioLDridiDBarbaraGMerliCCeteraGVercelliniP

Appl Biochem Biotechnol 197 , 51865199 (2025)

[DOI] [PubMed] [Google Scholar] 43.Dittrich AM, Meyer HA, Krokowski M, et al

Suspension culture and differentiation into liver organoids For differentiation of hPSCs into organoids, cells were harvested by incubation with Accutase (Life Technologies) for 10 min at 37 C until the cells had detached

After TEV digestion overnight at 4 C, the elution was then subjected to Strep-Tactin Sepharose resin (IBA, 2-1201-010), washed with wash buffer and eluted with wash buffer supplemented with 10 mM desthiobiotin (Sigma-Aldrich, D1411)