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Plates were incubated at 37 C for 2.5 h with gentle shaking, and then centrifuged at 2500 rpm, The supernatant was discarded, and the metabolic activity of cells was estimated with the 2.3-bis(2-methoxy-4-nitro-5-sulfophenyl)2H-tetrazolium-5-carboxanilide-inner salt (XTT

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Monitor for any unexpected effects

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Molecular and clinical prodrome of Parkinson disease: implications for treatment