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GSH is a tripeptide synthesized from glutamate, cysteine, and glycine in two steps catalyzed by -l-glutamyl-l-cysteine:glycine ligase and glutathione synthetase

It can also measure the spontaneous signal of networks within the brain during a resting state

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However, the natural product T2 , with a replacement of the sulfur atom of T1 by an oxygen atom, shows 713-fold decrease in the inhibitory ability (IC 50 = 378.11 M)