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In the aforementioned experiments, we observed that EA activated NTS GLP1 and concurrently increased the excitability of vagal afferent fibers, indicating EAs dual action on both central GLP-1 neurons and peripheral vagal inputs

Immunofluorescence labeling of HSD3B in rat testes and Leydig cells Paraformaldehyde (PFA, 4%)-fixed testis sections or isolated Leydig cells (LCs) were subjected to immunofluorescence staining of 3-hydroxysteroid dehydrogenase (HSD3B)

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