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Notably, lignans exhibit anti-cancer activity through multiple regulatory pathways ( 3 Factors affecting the appropriate extraction methods for phenolic compounds Polyphenols comprise a broad array of chemical structures, resulting in varied chemical and physical properties ( Furthermore, extraction efficiency is highly sensitive to operational parameters such as pH, type of solvent employed, solvent-to-sample ratio, and duration of the extraction process ( 3.1 Extraction methods of phenolic compounds 3.1.1 Ultrasound-assisted extraction method Ultrasound-assisted extraction is a widely used and efficient technique for isolating phenolic compounds, offering high yields in a relatively short time ( The process relies on acoustic cavitation, wherein ultrasonic waves disrupt plant cell walls by inducing rapid expansion and contraction of solid surfaces ( Randia monantha , mango seed kernels, olive pomace, and pine needles ( Ultrasound-assisted extraction provides notable advantages for the extraction of polyphenols

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Feed Preparation: Feed medium of 12 liters for fermentation was prepared according to the components of Table 3 and sterilized

gnde 3 tatl ka (3*5=15 ml) la deildir

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