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Consent for publication We confirm that the manuscript has been read and approved by all named authors, and that there are no other persons who satisfied the criteria for authorship but are not listed

Then the cells were washed with the FACS staining buffer to remove the unbound antibodies, and subjected to intracellular staining of nuclear Foxp3 protein according to the manufacturers protocol

[DOI] [PubMed] [Google Scholar] 34.Duzel A., Vlainic J., Antunovic M., Malekinusic D., Vrdoljak B., Samara M., Gojkovic S., Krezic I., Vidovic T., Bilic Z., et al

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This would not be achieved if amino acid component is given in equivalent amount

(d) an ice pack is helpful at the injection site if local reactions occur like edema