The following shRNA constructs were used (See also Key Resources Table): shGFP, shFOXO4-1: TRCN0000039720, Mature sequence: CCAGCTTCAGTCAGCAGTTAT, shFOXO4-2: TRCN0000039721, Mature sequence: CGTCCACGAAGCAGTTCAAAT, shFOXO4(mouse): TRCN0000071560, Mature sequence: GATCTGGATCTTGATATGTAT, shp53-1: TRCN0000003753, Mature sequence: CGGCGCACAGAGGAAGAGAAT and shp53-2: TRCN0000003754, Mature sequence: TCAGACCTATGGAAACTACTT
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The autism biomarkers consortium for clinical trials (ABC-CT): scientific context, study design, and progress toward biomarker qualification
Methods Study design and data source This retrospective cohort study used de-identified electronic medical records from the TriNetX global federated research network, which aggregates data from more than 140 healthcare institutions across 19 countries
One could also speculate that increased stress hormone activity (ie, catecholamines, cortisol) in the setting of anxiety or depression could have contributed to persistent hyperglycemia
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