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In summary, what makes All Carbon phases so powerful in long peptide purification is their versatility: Operate under both strong ion-pairing (TFA) and mild conditions (FA, neutral pH) Enable differential charge-based separation at higher pH ranges Provide surfaces that minimize peptide aggregation compared to silica phases Offer robust impurity clearance during both bulk removal (Stage 1) and fine polishing (Stage 2) By leveraging these properties, carbon media can address deletion impurities, partial deprotections, sequence isomers, charge variants, and aggregates collectivelyensuring a cleaner path toward regulatory-grade purity

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