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The mice received an intraperitoneal administration of isotonic saline (0.5 ml) to compensate for potential fluid loss

Peripheral GLP-1 or GLP-1RAs may interact with appetite-regulating regions of the brain via gaps in the blood-brain barrier, via tanycyte uptake, or indirectly via the nodose ganglia

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GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation