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Cell line culture GLUTag cells (a kind gift from Dan Drucker, Toronto) were maintained in Matrigel-coated flasks and low-glucose DMEM supplemented with 10% FBS, 100 units ml 1 penicillin and 0.1 mg ml 1 streptomycin

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Tirzepatide, a dual GIP/GLP-1 receptor agonist, has demonstrated greater efficacy in clinical trials, including the head-to-head SURMOUNT-5 trial comparing it to semaglutide 2.4 mg, potentially producing more substantial weight loss than traditional GLP-1 agonists alone

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PMID: 98 NakamuraK.Aoyama-IshiwatariS.NagaoT.PaaranM.ObaraC