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Ischemia-Reperfusion Protocol A separate group of hearts were excised as described above and exposed to 30/30 min of global, no-flow ischemia and reperfusion under control conditions (n = 7) or in the presence of 64M 4-ClDzp (n = 5)

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The consequences of this difference have been recognised in the investigation of the toxic mechanisms associated with gout, where the concentrations of UA that induce peripheral microcrystallisation and its related inflammatory reaction are not easily reached in rodents

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