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For prescription topical formulations used consistently, initial improvements in texture, hydration, and radiance typically appear at 2 to 4 weeks, meaningful visible changes to firmness and fine lines at 4 to 8 weeks, maximum early results at 8 to 12 weeks, and full cumulative benefit at 3 to 6 months of consistent daily use

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Separation was carried out using a non-isocratic method (20% - 50% methanol over 30 min) at 37C and a flow rate of 0.3 mL/min

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