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Sinks were dried at 40 C and stored in a desiccator until extraction

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146,147,148 Tuberous sclerosis is an autosomal dominant disorder arising from heterozygous mutations in the TSC1 and TSC2 genes that is commonly associated with deficits in long-term and working memory, intellectual disability, and ASD

This conversation reframes exercise not as punishment or weight loss driven, but as a powerful tool for resilience, independence, and quality of life

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Briefly, flash-frozen tissue samples were homogenized in ice-cold PBS containing 2 mM EDTA (1 ml PBS/EDTA per 10 mg tissue) using a Dounce homogenizer