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Confocal microscopy (Leica SP8), scanning electron microscopy (SEM, JEOL JSM-6610LV), transmission electron microscopy (TEM, JEM-1400 Flash), and atomic force microscopy (AFM, Bruker Dimension Icon) were employed to analyze the grafts surface and ultrastructure

Neurosci Lett 419(3):201205

RNA isolation and quantitative RT-PCR Total RNA from cells was isolated with Trizol reagent (Qiagen GmbH, Hilden, Germany) and RT-PCR was conducted using a quantitative SYBR Green RT-PCR kit (Applied Biosystems, Warrington, UK) was used with a Step One Plus RT-PCR System (Applied Biosystems)

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Liver plates merge imperceptible with surrounding hepatic parenchyma