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The digest was filtered through a 70-m mesh and centrifuged using a 28.7% Nycodenz gradient (Nycomed PharmaAS, Oslo, Norway)

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[DOI] [PMC free article] [PubMed] [Google Scholar] 74.Gabe MBN, Breitschaft A, Knop FK, Hansen MR, Kirkeby K, Rathor N, Adrian CL

The encapsulation efficiency of DNA into the LNP was determined by the Picogreen assay (Thermo Ficher Scientific) following the manufacturers protocol

The definition of metabolic syndrome is low HDL [high-density lipoprotein cholesterol], high triglycerides, obesity, hypertension, and diabetes