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siRNA-mediated knockdown of Nrf2 and CYPOR For transfection of HepG2 in (an individual well) of a 6-well plate (5 10 5 cells/well), solution A, consisting of 4 l Lipofectamine (Life Technologies) and 150 l Opti-MEM (Life Technologies) were mixed

The combination of N-terminal amine, histidine at position 2, and intervening amide nitrogen is a recurrent high-affinity copper-binding motif in peptide biochemistry the same general motif also appears in human serum albumin's N-terminal copper-binding site

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