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Quantitative RT-PCR was performed in triplicate using SYBR Green Supermix (Bio-Rad) on CFX96 real-time system (C1000 Thermal Cycler

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Fourteen genes whose expression is significantly correlated with regional emphysema severity or transcription factors that are highly connected to these genes in the relevance network were selected for quantitative RT-PCR validation in a subset of tissue cores from subjects with severe emphysema (see Additional file 1 for methods)

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