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The amount of melanin in your irises, and thus your eye color, is determined by your genes

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SLO activity was determined for each batch based on hemolytic activity, where one unit is defined as the amount of SLO required to lyse 50% of uninfected erythrocytes at 2% hematocrit in PBS for 30 min at 37 C, as determined by titration

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Sleep quality and duration should be assessed, as inadequate sleep disrupts metabolic hormones and increases appetite